Reporter
UAS-LacZ

Part:BBa_K758005:Design

Designed by: Takaaki Takeda   Group: iGEM12_KIT-Kyoto   (2012-09-18)


UAS LacZ, this part expresses LacZ in the presence of GAL4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3393
    Illegal PstI site found at 4
    Illegal PstI site found at 247
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3393
    Illegal PstI site found at 4
    Illegal PstI site found at 247
    Illegal NotI site found at 3447
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3393
    Illegal BglII site found at 377
    Illegal BamHI site found at 3614
    Illegal XhoI site found at 3454
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3393
    Illegal PstI site found at 4
    Illegal PstI site found at 247
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3393
    Illegal PstI site found at 4
    Illegal PstI site found at 247
    Illegal AgeI site found at 3519
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3501


Design Notes

UAS of this part is equivalent to the one of BBa_K758003. And LacZ of this part and BBa_J33202 is not the same one, but the role of LacZ of these parts are essentially the same.


Source

UAS, LacZ, and SV40 polyadenylation signal

References